Alkaline hydrolysis of poly(γ‐glutamic acid) produced by microorganism

The structure of ribonucleic acid. I. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysis.

Hydrolysis of S-substituted monoesters of phosphorothioic acid by alkaline phosphatase from Escherichia coli.

Alkaline phosphatase from Escherichia coli catalyzes the hydrolysis of S-substituted monoesters of phosphorothioic acid of the type RSP03Naz (R = -CH2CH2NH2,-CH2CHzNHCOCH3,-CH,COOH, or -CH2CHzCOOCzH~), at the S-P bond, to yield orthophosphate and the corresponding thioalcohols. The rate of enzymic hydrolysis of cysteamine S-phosphate was measured at different pH values and substrate concentrati...

Specificity of hydrolysis of phytic acid by alkaline phytase from lily pollen.

Phytases are the primary enzymes responsible for the hydrolysis of phytic acid, myo-inositol-1,2,3,4,5,6-hexakisphosphate (I-1,2,3,4,5,6-P6). A number of phytases with varying specificities, properties, and localizations hydrolyze phytic acid present in cells. The specificity of hydrolysis of phytic acid by alkaline phytase from lily (Lilium longiflorum L.) pollen is described. Structures of th...

Alkaline Hydrolysis of N-acetylolycine

A faster reaction of the cation is expected for the compounds with a free amino group in the acyl moiety. Such an increase was observed for Ala-Ala-pnitroan~lide b.ut not with Ala-p-nitroanilide. Fischer · et al. concluded that a larger inc-rease in k1 with the latter compound was compensated by a reductionin k2/ki. so that the observed k1k2/(k~1 + k2) term remains the same. This assumption of ...

Catalysis of the Hydrolysis of Phosphorylated Pyridines by Alkaline Phosphatase

Bacterial alkaline phosphatase is an active catalyst for the hydrolysis of N-phosphorylated pyridines, with values of the second-order rate constant kmt/Km in the range 0.4-1.2 X lo6 M-1 s-l a t pH 8.0, 25 OC. There is little or no dependence of the rate on the pKa of the leaving group; the value of @le is 0 f 0.05, which may be compared with @le = -1 .O for the nonenzymic reaction. Phosphoryla...